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Image Search Results
Journal: The American Journal of Pathology
Article Title: Myeloid-Derived Lymphatic Endothelial Cell Progenitors Significantly Contribute to Lymphatic Metastasis in Clinical Breast Cancer
doi: 10.1016/j.ajpath.2019.07.006
Figure Lengend Snippet: Antibodies Used for Flow Cytometry and Immunofluorescence
Article Snippet: Species specificity Clone name Concentration, μg/mL IF Flow cytometry CD3 Abcam (Cambridge, UK) ab5690 Rabbit anti-human (P) N/A 2 N/A CD4 Abcam ab133616 Rabbit anti-human (P) N/A 1.4 N/A CD8 Santa Cruz Biotechnology (Dallas, TX) sc-7188 Rabbit anti-human (P) N/A 2 N/A CD11b R&D Systems (Minneapolis, MN) MAB16992 Mouse anti-human (M) 238439 5 N/A CD11b BioXCell (West Lebanon, NH) BE0007 Rat anti-mouse (M) M1/70 10 N/A CD14 Santa Cruz Biotechnology sc-1182 Mouse anti-human (M) UCH-M1 2 N/A CD18 Invitrogen (Carlsbad, CA) MA1810 Mouse anti-human (M) TS1/18.1.2.11.4 10 N/A CD38 R&D Systems MAB24041 Mouse anti-human (M) 240726 5 N/A CD56 Santa Cruz Biotechnology sc-106 Mouse anti-human (M) ERIC1 2 N/A CD68 Thermo Fisher (Waltham, MA)
Techniques: Flow Cytometry, Concentration Assay
Journal: The American Journal of Pathology
Article Title: Myeloid-Derived Lymphatic Endothelial Cell Progenitors Significantly Contribute to Lymphatic Metastasis in Clinical Breast Cancer
doi: 10.1016/j.ajpath.2019.07.006
Figure Lengend Snippet: Tumor myeloid-derived lymphatic endothelial cell progenitors (M-LECP) are a subset of M2-type macrophages. Tumor sections were costained with antibodies against lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1) and CD204 (A), CD209 (B), or CD163 (C), typically used to identify M2-type tumor-associated macrophages. Circular arrangement of M-LECP cells [LYVE-1 and CD204/CD209 dual-positive clusters, indicated by white dashed line (A and B)] is reminiscent of nascent formation of a lymphatic vessel. Spatial organization of M-LECPs costained with other macrophage markers was observed in multiple tumor samples. White boxed areas in images in the third panels are shown in higher magnification in the fourth panels. Nuclei in merged images are identified by Hoechst stain. Scale bar = 100 μm. Original magnification: ×400 (A–C, first, second, and third panels); ×800 (A–C, fourth panels).
Article Snippet: Species specificity Clone name Concentration, μg/mL IF Flow cytometry CD3 Abcam (Cambridge, UK) ab5690 Rabbit anti-human (P) N/A 2 N/A CD4 Abcam ab133616 Rabbit anti-human (P) N/A 1.4 N/A CD8 Santa Cruz Biotechnology (Dallas, TX) sc-7188 Rabbit anti-human (P) N/A 2 N/A CD11b R&D Systems (Minneapolis, MN) MAB16992 Mouse anti-human (M) 238439 5 N/A CD11b BioXCell (West Lebanon, NH) BE0007 Rat anti-mouse (M) M1/70 10 N/A CD14 Santa Cruz Biotechnology sc-1182 Mouse anti-human (M) UCH-M1 2 N/A CD18 Invitrogen (Carlsbad, CA) MA1810 Mouse anti-human (M) TS1/18.1.2.11.4 10 N/A CD38 R&D Systems MAB24041 Mouse anti-human (M) 240726 5 N/A CD56 Santa Cruz Biotechnology sc-106 Mouse anti-human (M) ERIC1 2 N/A CD68 Thermo Fisher (Waltham, MA)
Techniques: Derivative Assay, Staining
Journal: Bioactive Materials
Article Title: Micropatterns and peptide gradient on the inner surface of a guidance conduit synergistically promotes nerve regeneration in vivo
doi: 10.1016/j.bioactmat.2021.07.010
Figure Lengend Snippet: (a) Merged immunofluorescence staining images of S100β (green), NF200 (red), and DAPI (blue) for SCs, axons and cell nuclei in proximal parts of newborn nerves post surgery for 9 and 14 d, respectively. (b) Merged immunofluorescence staining images of CD163 (green), CD86 (red), and DAPI (blue) for M2, M1 and cell nuclei in the proximal segments of regenerated nerves post surgery for 9 and 14 d, respectively. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Article Snippet: After fixed in formalin for 3 d, they were embedded by paraffin for immunofluorescence staining of NF200 (mouse anti-rat, 1:500; Peprotech Co., UK), S100β (rabbit anti-rat, 1:500; Peprotech Co., UK), CD86 (mouse anti-rat, 1:500; Peprotech Co., UK),
Techniques: Immunofluorescence, Staining